Not surprise he didn't make it but I was hoping that any other anime fan might vote for him. He's such a delightfully evil villain and the only recurring villain in the Jojo's Bizarre Adventure franchise. Even after his death his shadow looms over the series (even appearing in the rebooted universe in a non-vampire form). Plus, he's named after Ronnie James Dio.
Definitely the biggest omission for me on my list, also a huge fan of Ronnie James Dio. Big fan of Jojo, I guess my brain just forgot Dio is a vampire. I remembered him more as a guy who can stop time and punch steam rollers. I guess I could've included Kars and whatnot too.

(The Cars is another fine band) Or maybe Kars was an immortal pillar man not a mask made vampire, that origin stuff kinda blurs together.
Great fun countdown, gotta love all those damn vampires. To paraphrase that guy from the end of The Lost Boys.
Here's my list:
01. D (Vampire Hunter D)
02. David (The Lost Boys)
03. Spike (Buffy the Vampire Slayer)
04. Dracula (especially Christopher Lee)
05. Angel (Buffy the Vampire Slayer)
06. Peter Leow (Vampire's Kiss)
07. Mr. Trick (Buffy the Vampire Slayer)
08. Count von Count (Sesame Street)
09. Count Chocula
10. Darla (Buffy the Vampire Slayer)
11. Harmony (Buffy the Vampire Slayer)
12. Marko (The Lost Boys)
13. The Master (Buffy the Vampire Slayer)
14. Duckula
15. Bill Compton (True Blood)
16. Drusilla (Buffy the Vampire Slayer)
17. Rachel (Vampire's Kiss)
18. Jessica Hamby (True Blood)
19. Alucard (Castlevania: Symphony of the Night)
20. Diamond (Bastard!!)
21. Devlin Waugh (Devlin Waugh)
22. Strahd von Zarovich (Dungeons & Dragons)
23. Vampirella (Vampirella)
24. Chastity (Chastity)
25. Sam (Lost Boys: The Tribe)